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Project Information

INVESTIGATION OF FACTORS AFFECTING BIOAEROSOL COLLECTION AND GENERATION

Agency:
NIFA

USDA/National Institute of Food and Agriculture

Project Number:
1002274
Contact PI / Project Leader:
MAINELIS, G, .
Awardee Organization:
RUTGERS THE ST UNIV OF NJ NEW BRUNSWICK

Description

Abstract Text:
Investigation of the presence of airborne microorganisms (bioaerosols) in the ambient air is of interest due to their environmental and human health effects. In the past few years, quantitative polymerase chain reaction (qPCR) has gained popularity in bioaerosol research due to its capacity to rapidly quantify and identify microorganisms in air samples. However, there are indications that air sampling may lead to the loss of structural integrity of bacteria thus affecting the ability of qPCR to provide accurate and reliable data. in addition, stable and reliable bioaerosol generation is another important element of bioaerosol research. While there have been advances in the development of bioaerosol generators, there is still a need for bioaerosol generators which produce high bioaerosol concentrations with minimal effects on microorganism culturability and structural integrity and are easy to operate. Any potential bioaerosol damage occurring during aerosolization would be transferred to collected bioaerosol samples. Thus, it is important to investigate the effects of aerosolization on bioaerosols, including potential effects on their membrane integrity.Thus this research proposes a two-prong effort to improve our ability to detect and measure airborne biological agents: 1) by investigating the effect of sampling on the integrity of biological particles and, as a result, our ability to detect and quantify biological particles; and 2) to investigate the performance of common and new bioaerosol generators on the viability and integrity of bioaerosols and application of those generators in bioaerosol studies.
Project Terms:
Affect; Air; air sampling; Bacteria; Biological; Biological Products; Collection; Data; Development; Elements; Generations; Health; Human; improved; interest; Investigation; Lead; Measures; Membrane; microorganism; particle; Performance; Polymerase Chain Reaction; Research; Sampling

Details

Contact PI / Project Leader Information:
Name:  MAINELIS, G, .
Other PI Information:
Not Applicable
Awardee Organization:
Name:  RUTGERS THE ST UNIV OF NJ NEW BRUNSWICK
City:  NEW BRUNSWICK    
Country:  UNITED STATES
Congressional District:
State Code:  NJ
District: 
Other Information:
Fiscal Year: 2014
Award Notice Date:
DUNS Number: 001912864
Project Start Date: 01-Feb-2014
Budget Start Date:
CFDA Code: 10.203
Project End Date: 31-Jan-2019
Budget End Date:
Agency: ?

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USDA/National Institute of Food and Agriculture
Project Funding Information for 2014:

No funding information available for Project Number: 1002274

Results

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