Skip Navigation Links

Project Information

ROLE OF NUCLEAR IGFBP-3 IN STRESS-INDUCED APOPTOSIS IN BOVINE MAMMARY EPITHELIAL CELLS

Agency:
NIFA

USDA/National Institute of Food and Agriculture

Project Number:
2009-35206-05210
Contact PI / Project Leader:
COHICK, WENDIE S
Awardee Organization:
RUTGERS THE ST UNIV OF NJ NEW BRUNSWICK

Description

Abstract Text:
Milk yield is a direct reflection of the number of milk-producing cells in the udder at any one time. The gradual decline in daily milk yield that occurs in dairy cows over a lactation cycle is due to loss of these cells through cell death. If this process can be prevented, the efficiency of milk production will be improved. This project is based on the idea that stress alters the physical interaction between three pre-existing cellular proteins, causing two of them to move to different locations in the cell where they induce cell death. These proteins are IGFBP-3, a protein that is involved in cell death through unknown mechanisms, and two nuclear receptors Nur77 and retinoic X receptor-alpha (RXR-alpha), which have been shown to move to the mitochondria and induce cell death under certain conditions. The specific objectives will be to determine if IGFBP-3, Nur77 and/or RXRalpha are necessary for cell death in response to stress in milk-producing cells, to determine how these three molecules physically interact in the nucleus during stress, and to determine if specific chemical pathways in the cell are involved in mediating these interactions. The expected result is that under normal conditions, IGFBP-3 will be found in the nucleus in a complex with the nuclear receptors Nur77 and RXRalpha. During stress, these proteins will be modified by chemical signals so that IGFBP-3 will no longer hold Nur77 and RXRalpha in the nucleus, allowing them to move to the mitochondria where they induce cell death. Information gained from these studies will fill critical gaps in our knowledge of how cell death is regulated in milk-producing cells.
Project Terms:
Apoptosis; base; Breast Epithelial Cells; Cattle; Cell Death; Cell Nucleus; Cells; Chemicals; Complex; Heat shock proteins; improved; Insulin-Like Growth Factor Binding Protein 3; Knowledge; Lactation; Location; Mediating; Milk; Mitochondria; Nuclear; Nuclear Receptors; Pathway interactions; prevent; Process; Production; Proteins; receptor; response; Role; Signal Transduction; Stress; stress protein; Time; Udders

Details

Contact PI / Project Leader Information:
Name:  COHICK, WENDIE S
Other PI Information:
Not Applicable
Awardee Organization:
Name:  RUTGERS THE ST UNIV OF NJ NEW BRUNSWICK
City:  NEW BRUNSWICK    
Country:  UNITED STATES
Congressional District:
State Code:  NJ
District:  06
Other Information:
Fiscal Year: 2008
Award Notice Date:
DUNS Number: 001912864
Project Start Date: 01-Sep-2013
Budget Start Date:
CFDA Code: 10.212
Project End Date: 31-Aug-2015
Budget End Date:
Agency: ?

Agency: The entity responsible for the administering of a research grant, project, or contract. This may represent a federal department, agency, or sub-agency (institute or center). Details on agencies in Federal RePORTER can be found in the FAQ page.

USDA/National Institute of Food and Agriculture
Project Funding Information for 2008:
Year Agency

Agency: The entity responsible for the administering of a research grant, project, or contract. This may represent a federal department, agency, or sub-agency (institute or center). Details on agencies in Federal RePORTER can be found in the FAQ page.

FY Total Cost
2008 NIFA

USDA/National Institute of Food and Agriculture

$349,963

Results

i

It is important to recognize, and consider in any interpretation of Federal RePORTER data, that the publication and patent information cannot be associated with any particular year of a research project. The lag between research being conducted and the availability of its results in a publication or patent award varies substantially. For that reason, it's difficult, if not impossible, to associate a publication or patent with any specific year of the project. Likewise, it is not possible to associate a publication or patent with any particular supplement to a research project or a particular subproject of a multi-project grant.

ABOUT FEDERAL REPORTER RESULTS

Publications: i

Click on the column header to sort the results

PubMed = PubMed PubMed Central = PubMed Central Google Scholar = Google Scholar

Patents: i

Click on the column header to sort the results

Similar Projects

Download Adobe Acrobat Reader:Adobe Acrobat VERSION: 3.41.0 Release Notes
Back to Top