Skip Navigation Links

Project Information

VITAMIN A AND CAROTENOID METABOLISM DURING PREGNANCY AND DEVELOPMENT

Agency:
NIFA

USDA/National Institute of Food and Agriculture

Project Number:
0205611
Contact PI / Project Leader:
QUADRO, L.
Awardee Organization:
RUTGERS THE ST UNIV OF NJ NEW BRUNSWICK

Description

Abstract Text:
To carry out the experiments in objective 1, ML-/-R-/- females be mated with ML-/-R-/- males. At the time of the plug (0.5dpc), the females will be maintained on a vitamin A-sufficient (25 IU/g), or vitamin A-deficient (<0.22 IU/g) or vitamin A-excess diet (220 IU/g). Wild-type, RBP-/- and ML-/- females mated with wild-type, RBP-/- and ML-/- males, respectively, will be used as controls on the same diets during pregnancy. At 14.5dpc, embryos will be collected, genotyped and examined both for their external morphology and by HPLC analysis. Their retinoid status will be also assessed by analyzing the expression levels of LRAT, Stra6, RALDH2 and Cyp26A1 mRNA by real-time PCR. Maternal serum and tissues and placenta will be also collected and analyzed by HPLC. In addition, wild-type, RBP-/-, ML-/- and ML-/-R-/- dams at 14.5 dpc will be fed by gavage with a single dose of radiolabeled retinol (2x106 cpm all-trans[3H]retinol and 6ug of unlabeled all-trans-retinol in a peanut oil). Subsequently, the dams will be sacrificed 1, 5, 10 and 24 hours after the injection. Total [3H]retinoid in maternal and embryonic tissues will be measured by a scintillation counter and HPLC will be also performed on the same tissues to analyze the distribution of retinol and retinyl ester and [3H]retinoid. To carry out the experiment in objective 2, CMO I-/-RBP-/- females will be mated with CMO I+/-RBP-/- males. At 0.5dpc, the females will be placed on a vitamin A-deficient diet and will be administered B-carotene according to one of the following regimens: #1. from 0.5 to 14.5dpc (throughout gestation); #2. from 7.5 to 9.5dpc; #3. from 7.5 to 14.5dpc. A single daily dose of B-carotene (10microg/g of body weight) will be administered to the dams by IP injection. Pregnant females will be sacrificed at 14.5dpc, and embryos will be collected and examined both for their external morphology and in histological sections. Maternal serum, liver, lung and adipose tissue, placenta, yolk sac and whole embryo will be collected to perform retinol, retinyl ester and carotenoids analysis by HPLC. To carry out the experiments in objective 3, wild-type, LDLr knockout (LDLr-/-), SR-BI knockout (SR-BI-/-) and ML-/- mice will be used. LDLr-/- and ML-/- females, maintained on a vitamin A-sufficient diet, will be mated with LDLr+/- and ML+/- males, respectively. Since SR-BI-/- females are not fertile, SR-BI+/- females will be mated with SR-BI-/- males. At 10.5, 12.5, 14.5 and 16.5dpc dams will be supplemented by IP injection with a single dose of radiolabeledB-carotene (106 cpm and 10microg of B-carotene/g of body weight). Dams will be sacrificed 1, 5, 10 and 24 hours after the injection. Wild-type preganant females injected with radiolabeled B-carotene will serve as controls. Maternal and embryonic tissues will be analyzed for their labeled and unlabeled B-carotene content by simultaneous analysis of carotenoids by HPLC analysis and radiolabeled carotenoids counts. B-carotene metabolites will also be identified by GC-MS and LC-MS analysis.
Project Terms:
Adipose tissue; All-Trans-Retinol; Arachis hypogaea; Body Weight; Carotene; Carotenoids; corticosterone methyl oxidase I; Development; Diet; Dose; Embryo; embryo tissue; Esters; feeding; Female; Genotype; High Pressure Liquid Chromatography; Hour; Injection of therapeutic agent; Intraperitoneal Injections; Knock-out; Label; Liver; Lung; male; maternal serum; Measures; Messenger RNA; Metabolism; Morphology; Mus; Oils; Partner in relationship; Peanuts - dietary; Placenta; Pregnancy; pregnant; Radiolabeled; radiotracer; Regimen; research study; Retinoids; Scintillation Counter; Time; Tissues; Vitamin A; Yolk Sac

Details

Contact PI / Project Leader Information:
Name:  QUADRO, L.
Other PI Information:
Not Applicable
Awardee Organization:
Name:  RUTGERS THE ST UNIV OF NJ NEW BRUNSWICK
City:  NEW BRUNSWICK    
Country:  UNITED STATES
Congressional District:
State Code:  NJ
District: 
Other Information:
Fiscal Year: 2009
Award Notice Date:
DUNS Number: 001912864
Project Start Date: 01-Oct-2008
Budget Start Date:
CFDA Code: 10.203
Project End Date: 30-Sep-2013
Budget End Date:
Agency: ?

Agency: The entity responsible for the administering of a research grant, project, or contract. This may represent a federal department, agency, or sub-agency (institute or center). Details on agencies in Federal RePORTER can be found in the FAQ page.

USDA/National Institute of Food and Agriculture
Project Funding Information for 2009:

No funding information available for Project Number: 0205611

Results

i

It is important to recognize, and consider in any interpretation of Federal RePORTER data, that the publication and patent information cannot be associated with any particular year of a research project. The lag between research being conducted and the availability of its results in a publication or patent award varies substantially. For that reason, it's difficult, if not impossible, to associate a publication or patent with any specific year of the project. Likewise, it is not possible to associate a publication or patent with any particular supplement to a research project or a particular subproject of a multi-project grant.

ABOUT FEDERAL REPORTER RESULTS

Publications: i

Click on the column header to sort the results

PubMed = PubMed PubMed Central = PubMed Central Google Scholar = Google Scholar

Patents: i

Click on the column header to sort the results

Similar Projects

Download Adobe Acrobat Reader:Adobe Acrobat VERSION: 3.41.0 Release Notes
Back to Top