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Project Information

COMPETITIVE EQUILIBRIUM NUCLEIC ACID BIOSENSOR

Agency:
NSF

National Science Foundation

Project Number:
1033788
Contact PI / Project Leader:
BRESLAUER, KENNETH J.
Awardee Organization:
RUTGERS THE ST UNIV OF NJ NEW BRUNSWICK

Description

Abstract Text:
While there are many methods and devices for detection of nucleic acids in vitro and in vivo, no general solution has emerged that provides simple, rapid, nucleotide sequence selective quantification of nucleic acids. A highly sensitive, highly selective nucleic acid biosensor based on a competitive equilibrium between a probe and a target nucleic acid in conjunction with a newly developed instrument will be developed. The nucleic acid biosensor, founded on a novel thermodynamic formalism for describing the interactions of a duplex probe with its target, provides means for sensitive, highly selective quantification of the target.
Successful development of the competitive equilibrium nucleic acid biosensor will provide scientists with the means to detect and quantify, at high sensitivity, any nucleic acid with single base nucleotide sequence selectivity. Further development of sensitive and highly specific nucleic acid biosensors is critical for fully exploiting the rich genomic information resulting from the sequencing of the genomes of myriad microorganisms, plants and animals, including humans. In addition, recent discoveries about the regulation of gene expression by small ribonucleic acid molecules operating at the level of messenger RNA have vastly increased the number and variety of nucleic acids that must be identified, detected and quantified if one is to understand, and potentially modulate, a range of critical biological processes; including, but not limited to development, apoptosis, stress response and differentiation. The nucleic acid biosensor developed here will yield the requisite experimental capacity to detect and characterize biological targets for diagnostic applications, as well as for basic knowledge/understanding of biological regulatory mechanisms.
Project Terms:
Animals; Apoptosis; base; Base Sequence; Biological; biological adaptation to stress; Biological Process; Biosensor; Development; Devices; Diagnostic; Equilibrium; Gene Expression Regulation; genome sequencing; Genomics; Human; In Vitro; in vivo; instrument; Knowledge; Messenger RNA; Methods; microorganism; novel; nucleic acid detection; Nucleic Acids; Plants; RNA; Scientist; Solutions; Thermodynamics

Details

Contact PI / Project Leader Information:
Name:  BRESLAUER, KENNETH J.
Other PI Information:
SARVAZYAN, ARMEN; PLUM, GEORGE
Awardee Organization:
Name:  RUTGERS THE ST UNIV OF NJ NEW BRUNSWICK
City:  NEW BRUNSWICK    
Country:  UNITED STATES
Congressional District:
State Code:  NJ
District:  06
Other Information:
Fiscal Year: 2010
Award Notice Date: 03-Aug-2010
DUNS Number: 001912864
Project Start Date: 15-Sep-2010
Budget Start Date:
CFDA Code: 47.041
Project End Date: 31-Aug-2013
Budget End Date:
Agency: ?

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National Science Foundation
Project Funding Information for 2010:
Year Agency

Agency: The entity responsible for the administering of a research grant, project, or contract. This may represent a federal department, agency, or sub-agency (institute or center). Details on agencies in Federal RePORTER can be found in the FAQ page.

FY Total Cost
2010 NSF

National Science Foundation

$350,003

Results

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