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Project Information

CAREER: IRON-SULFUR CLUSTER ASSEMBLY IN BACILLUS SUBTILIS

Agency:
NSF

National Science Foundation

Project Number:
1750624
Contact PI / Project Leader:
BOYD, JEFFREY M
Awardee Organization:
RUTGERS THE ST UNIV OF NJ NEW BRUNSWICK

Description

Abstract Text:
All forms of life require that many of their proteins have non-protein parts known as cofactors added to them so that they can function properly. Many of these cofactors are built from vitamins and minerals that are important in the diet. One such cofactor is the iron-sulfur (Fe-S) cluster, which allows proteins to perform many important types of chemistry. Because free Fe and S are toxic to cells, elaborate protein systems are used to build these cofactors. Once they are built, the Fe-S clusters must be trafficked to, and inserted into, proteins that require them. Cells that cannot properly build Fe-S clusters or maturate Fe-S proteins have chronic complications, which often results in cell death. The nature of this cellular machinery, however, is not well understood, and many questions remain about how organisms build Fe-S clusters and deliver them to proteins that require them for function. Novel Fe-S protein maturation proteins continue to be discovered, highlighting the fact that a global understanding of this process is lacking. The studies to be conducted in this project will impact the understanding of iron-dependent processes from bacteria to humans. The project will also provide unique educational opportunities for two postdoctoral scholars and up to ten undergraduate students. These trainees will be technically and intellectually trained and will receive the necessary guidance and resources to aid their transition into the next phases of their careers. The trainees will focus on enhancing their communication skills, technical skills, teaching skills, and personnel management skills.The Gram-positive bacterium Bacillus subtilis utilizes the SufCDSUB Fe-S cluster biosynthetic system and at least two Fe-S cluster carriers. Additional factors have been identified that have roles in Fe-S protein maturation, including SufT and YlaN; however, the functions of these molecules are unknown. Moreover, the Fe and electron donors for Suf-directed Fe-S cluster synthesis, as well as additional carrier molecules, are unknown. To properly understand how Fe-S proteins are maturated, the components involved must be identified and their functions defined. This project combines classic genetics and biochemistry with state-of-the-art, next-generation technologies and computational biology to 1) determine the physical interactions between SufT, YlaN, and Fe-S cluster synthesis, assembly, and target proteins, 2) quantify the necessity of SufT and alternate Fe-S cluster assembly factors for the function of specific metabolic pathways that require Fe-S enzymes, 3) assign biochemical functions to SufT and YlaN, and 4) model and predict the effect of faulty Fe-S protein maturation and validate the effects in vivo.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
Project Terms:
Award; Bacillus subtilis; Bacteria; Biochemical; Biochemistry; career; Cell Death; Cells; Chemistry; Chronic; cofactor; Communication; Computational Biology; Diet; Educational process of instructing; electron donor; Enzymes; Evaluation; Foundations; Genetic; Gram-Positive Bacteria; Human; in vivo; Iron; Life; Metabolic Pathway; Minerals; Mission; Modeling; Names; Nature; next generation; novel; Organism; Personnel Management; Phase; Process; Proteins; Resources; Role; skills; Sulfur; System; Technical Expertise; Technology; Training; undergraduate student; Vitamins

Details

Contact PI / Project Leader Information:
Name:  BOYD, JEFFREY M
Other PI Information:
Not Applicable
Awardee Organization:
Name:  RUTGERS THE ST UNIV OF NJ NEW BRUNSWICK
City:  PISCATAWAY    
Country:  UNITED STATES
Congressional District:
State Code:  NJ
District:  06
Other Information:
Fiscal Year: 2018
Award Notice Date: 29-May-2018
DUNS Number: 001912864
Project Start Date: 01-Jun-2018
Budget Start Date:
CFDA Code: 47.074
Project End Date: 31-May-2023
Budget End Date:
Agency: ?

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National Science Foundation
Project Funding Information for 2018:
Year Agency

Agency: The entity responsible for the administering of a research grant, project, or contract. This may represent a federal department, agency, or sub-agency (institute or center). Details on agencies in Federal RePORTER can be found in the FAQ page.

FY Total Cost
2018 NSF

National Science Foundation

$174,873

Results

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